Spotlight is a cross-platform GUI-based software package designed to perform image analysis on sequences of images generated by combustion and fluid physics experiments run in a microgravity environment. Spotlight can perform analysis on a single image in an interactive mode or perform analysis on a sequence of images in an automated fashion. Image processing operations can be employed to enhance the image before various statistics and measurement operations are performed. An arbitrarily large number of objects can be analyzed simultaneously with independent areas of interest. Spotlight saves results in a text file that can be imported into other programs for graphing or further analysis. Spotlight can be run on Microsoft Windows, Linux, and Apple OS X platforms. Klimek, Robert and Wright, Ted Glenn Research Center NASA/TM-2006-214084, E-15421 IMAGE ANALYSIS; IMAGE PROCESSING; GRAPHICAL USER INTERFACE; COMBUSTION PHYSICS; FLUID DYNAMICS; MICROGRAVITY; AUGMENTATION; UNIX (OPERATING SYSTEM); SEQUENCING; TEXTS