This second edition provides new and updated chapters describing the utility and attributes of different tools that are used for studying microbial systems. Chapters detail a number of methods, including in silico system level analyses by MetaFlux, Kbase genome-scale model builder, COBRA toolbox, NanoSIP, and PAMMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Microbial Systems Biology: Methods and Protocols, Second Edition aims to introduce and aid scientists in using the various tools that are currently available for analysis, modification, and utilization of microbial organisms.