Back to Search

Targeted Mutagenesis in Mice

AUTHOR Papaioannou, Virginia; Joyner, Alexandra L.; Pedersen, Roger A.
PUBLISHER Cold Spring Harbor Laboratory Press (01/01/1993)
PRODUCT TYPE Video (Video, VHS Format)

Description
Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. When these cells are genetically manipulated, this approach discloses much about the function of specific genes.

Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition (see page 22).

This video guide complements the above mentioned manual and Transgenic Techniques in Mice: A Video Guide (see below). It provides step-by-step demonstrations of the following procedures required for making mutant mice.

Demonstration 1: Starting embryonic stem (ES) cell lines

Demonstration 2: Making fibroblast feeder layers

Demonstration 3: Growing established ES cell lines

Demonstration 4: Targeting ES cell lines

Demonstration 5: Making chimeras with ES cells

Demonstration 6: Making a mouse

Show More
Product Format
Product Details
ISBN-13: 9780879694302
ISBN-10: 0879694300
Content Language: English
More Product Details
Carton Quantity: 0
Product Dimensions: 4.76 x 1.19 x 7.98 inches
Weight: 0.57 pound(s)
Country of Origin: US
Subject Information
BISAC Categories
Science | Life Sciences - Genetics & Genomics
Science | Research & Methodology
Dewey Decimal: 575
Descriptions, Reviews, Etc.
publisher marketing
Embryonic stem (ES) cells are important vehicles for transfer of genes into mice. When combined with normal pre-implantation embryos and transferred to foster mothers, ES cells contribute to the development of many different tissues, including the germline. When these cells are genetically manipulated, this approach discloses much about the function of specific genes.

Detailed methods for using ES cells are described in the manual Manipulating the Mouse Embryo: A Laboratory Manual, Second Edition (see page 22).

This video guide complements the above mentioned manual and Transgenic Techniques in Mice: A Video Guide (see below). It provides step-by-step demonstrations of the following procedures required for making mutant mice.

Demonstration 1: Starting embryonic stem (ES) cell lines

Demonstration 2: Making fibroblast feeder layers

Demonstration 3: Growing established ES cell lines

Demonstration 4: Targeting ES cell lines

Demonstration 5: Making chimeras with ES cells

Demonstration 6: Making a mouse

Show More
List Price $200.00
Your Price  $198.00
Video